Rabbit anti-Human CD300F/IREM-1 Monoclonal Antibody for Flow Cytometry - Q8TDQ1

Core Product Specifications and Parameters

Biological Background: CD300F/IREM-1 Function and Localization

• CD300F, also designated CMRF35-like molecule 1, IREM-1, or CLM-1, is an inhibitory receptor belonging to the CD300 family of leukocyte surface proteins. It is expressed as multiple isoforms due to alternative splicing.
• The protein functions as an inhibitory receptor on myeloid cells and mast cells, dampening immune activation signals. Related references: PMID:15549731
• CD300F recognizes externalized phosphatidylserine on apoptotic cells and promotes macrophage-mediated efferocytosis, contributing to immune homeostasis.
• Through binding to ceramide and sphingomyelin, it negatively regulates Fc epsilon RI-dependent mast cell degranulation and allergic inflammation. Related references: PMID:24035150
• CD300F suppresses Toll-like receptor (TLR) signaling by recruitment of SHP-1 and SHP-2 phosphatases, limiting MYD88- and TRIF-dependent pathways. Related references: PMID:22043923
• It may serve as an IL-4 co-receptor, enhancing IL-4/IL-13-induced STAT6 signaling, and also inhibits osteoclastogenesis.
• Subcellularly, CD300F is localized to the cell membrane and is predominantly expressed in monocytes, monocyte‑related cells, spleen, peripheral blood leukocytes, and lung.
• Post‑translational modifications include N‑glycosylation, phosphorylation, and disulfide bond formation, contributing to observed molecular weights of 18, 21, 26, and 32 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 20–156) targets the extracellular domain, making this antibody well suited for detecting surface CD300F by flow cytometry.
• As a direct conjugate, it enables single‑step staining without secondary antibodies, minimizing non‑specific binding.
• Because CD300F is selectively expressed on myeloid cells, consider using Fc receptor blocking and appropriate isotype controls to reduce background in monocyte‑ or macrophage‑rich samples.
• Validate expression in your specific cell type; while highest levels are reported in monocytes and monocyte‑derived cells, tissue‑specific expression patterns may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material procurement, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing for challenging targets. This product supports research into immune regulatory pathways, myeloid cell biology, and allergic disorders. With end‑to‑end technical and logistical support, we help diagnostic manufacturers and laboratories accelerate development from discovery to clinical application.