Rabbit anti-Human CD300F/IREM-1 Monoclonal Antibody for FC - Q8TDQ1

Core Product Specifications and Parameters

Biological Background: CD300F/IREM-1 Function and Localization

• CD300F/IREM-1 (CMRF35-like molecule 1) is a transmembrane glycoprotein belonging to the CD300 family of immune receptors, also known as CLM1, IgSF13, and NKIR.
• It functions as an inhibitory receptor on myeloid cells and mast cells. Related references: PMID:15549731
• Negatively regulates Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of SHP-1 and SHP-2 phosphatases. Related references: PMID:22043923
• Recognizes phosphatidylserine on apoptotic cells, promoting phagocytosis (efferocytosis) by macrophages while inhibiting it in dendritic cells, contributing to immune homeostasis.
• Inhibits Fc epsilon receptor-dependent mast cell activation and allergic responses by binding to ceramide and sphingomyelin lipids. Related references: PMID:24035150
• Highly expressed in spleen, peripheral blood leukocytes, monocytes, and lung; selectively found in monocytes and monocyte-related cells.
• Upon engagement, induces macrophage cell death, and may also act as a coreceptor for IL-4, augmenting IL-4/IL-13 signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 20-156), making this antibody suitable for detecting native surface-expressed CD300F/IREM-1 in live-cell flow cytometry.
• The conjugate provides bright red fluorescence; consider titrating the antibody to optimize staining index and minimize background.
• Multiple bands (18–32 kDa) are observed in western blotting due to glycosylation and isoform variations; confirm specificity with appropriate controls and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD300F/IREM-1-related research and assay development, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your projects from R&D to commercialization.