Rabbit anti-Human CD300c Monoclonal Antibody for IF/ICC, FC - Q08708

Core Product Specifications and Parameters

Biological Background: CD300c (CMRF35-like molecule 6) Function and Localization

• Encodes an immunoglobulin superfamily receptor, likely involved in immune modulation and signaling pathways.
• Expressed on the surface of monocytes, neutrophils, and subsets of peripheral blood T- and B-lymphocytes.
• Consists of an extracellular Ig-like domain, a single transmembrane helix, and a cytoplasmic tail, typical of cell surface receptors.
• Classified as a glycoprotein containing structural disulfide bonds, critical for domain stability.
• Subcellular localization is primarily the cell membrane, consistent with a signal peptide and transmembrane anchor.
• Belongs to the CD300 gene family, which includes activating and inhibitory receptors that fine-tune immune responses.
• Tissue distribution suggests roles in both innate and adaptive immunity, given its presence across diverse leukocyte lineages.

Experimental Guidance and Technical Tips

• Generated against the extracellular domain (aa 21-183), enabling detection of surface CD300c in live-cell applications like flow cytometry and IF/ICC.
• In FC, consider a live-cell staining protocol without fixation to preserve surface epitopes; validate with appropriate isotype controls.
• For IF/ICC, the target may be accessible without permeabilization; include permeabilized controls to assess intracellular localization if needed.
• Optimize antibody concentration and staining conditions for specific cell types, as CD300c expression varies among leukocyte subsets.
• Use CD300c-transfected cells or known positive cell lines as positive controls, and untreated cells or isotype controls as negative references.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers and research institutions, offering validated monoclonal antibodies like this anti-CD300c conjugate, along with antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. With expertise in immune receptor research, we support projects from early-stage development to clinical application, ensuring reliable supply continuity for your diagnostic and research needs.