Rabbit anti-Human CD300c Monoclonal Antibody for IF/ICC, FC - Q08708

Core Product Specifications and Parameters

Biological Background: CD300c Function and Localization

• CD300c (CMRF35-like molecule 6), encoded by the CD300C gene, is a type I transmembrane protein belonging to the CD300 family of leukocyte surface receptors.
• CD300c functions as an immune receptor implicated in modulating innate and adaptive immune responses through interactions with its ligands on target cells.
• It is part of the CD300 gene cluster on chromosome 17q25, which encodes receptors containing immunoglobulin-like domains that play diverse roles in immune cell regulation.
• The protein localizes to the cell membrane, consistent with its role in signal transduction upon ligand engagement.
• CD300c is expressed on the surface of monocytes, neutrophils, and subsets of peripheral blood T and B lymphocytes.
• Post-translational modifications include a signal peptide, disulfide bonds, and N-linked glycosylation, which are characteristic of immunoglobulin superfamily members.
• A three-dimensional structure of CD300c has been solved, shedding light on its extracellular domain architecture and potential ligand-binding surfaces.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21–183), making the antibody suitable for detecting surface CD300c by flow cytometry without permeabilization.
• The conjugate allows direct detection in both immunofluorescence (IF/ICC) and flow cytometry (FC); ensure proper compensation when multiplexing with other fluorophores.
• For IF/ICC, optimize fixation and permeabilization conditions, as the antibody targets a transmembrane protein; typical protocols using 4% PFA fixation followed by permeabilization with Triton X-100 or saponin may be suitable.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls in flow cytometry to assess background staining and gating boundaries.
• As the antibody is validated on human samples, perform a titration experiment to determine the optimal concentration for your specific cellular system and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supplying high-quality IVD raw materials to diagnostic manufacturers, laboratories, and research institutes worldwide. This Rabbit anti-Human CD300c mAb supports immune receptor studies, and we offer a comprehensive portfolio of monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary reagents. Our one-stop sourcing platform also provides access to rare target raw materials and tailor-made solutions, helping you streamline your development from concept to clinic.