Rabbit anti-Human CD300c Monoclonal Antibody for IF/ICC, FC - Q08708

Core Product Specifications and Parameters

Biological Background: CD300c (CMRF35-like molecule 6) Function and Localization

• Official protein name: CMRF35-like molecule 6, also known as CD300 antigen-like family member C, CMRF35-A1, and immunoglobulin superfamily member 16 (IgSF16).
• Belongs to the CD300 family of immune receptors, characterized by a single immunoglobulin-like domain.
• Predicted to be a single-pass type I transmembrane glycoprotein with a signal peptide and disulfide bonds.
• Subcellular location: Cell membrane.
• Tissue specificity: Expressed on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes, and lymphocytic cell lines.
• Associated keywords include immunity, receptor, and 3D-structure, suggesting a role in immune regulation.
• Predicted molecular weight (UniProt): 24830 Da; supplier-observed molecular weight: ~25 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 21-183), enabling recognition of surface-expressed CD300c.
• Direct conjugation allows one-step detection without secondary reagents in fluorescence-based applications.
• For flow cytometry, consider using fresh peripheral blood mononuclear cells (PBMCs) or monocytic cell lines; include Fc receptor blocking to reduce nonspecific binding.
• For immunofluorescence, optimized fixation and permeabilization conditions should be determined empirically, though surface staining may require only mild fixation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and services, supporting research in immune cell biology and leukocyte targets. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, tailored for diagnostic and research applications. For targets like CD300c, we facilitate procurement of high-quality antibodies and related reagents to streamline your assay development.