Rabbit anti-Human CD300a Monoclonal Antibody for Flow Cytometry - Q9UGN4

Core Product Specifications and Parameters

Biological Background: CD300a Function and Localization

• CD300a, also known as CMRF35-like molecule 8, IRp60, or CD300 antigen-like family member A, is an inhibitory receptor belonging to the immunoglobulin superfamily. It contains one Ig-like domain and two ITIM motifs.
• In natural killer (NK) cells, CD300a down-regulates cytolytic activity, and in mast cells, it down-regulates degranulation, contributing to immune inhibitory signaling. Related references: PMID:10746781 PMID:16339535 PMID:9701027
• CD300a negatively regulates Toll-like receptor (TLR) signaling mediated by MYD88, but not TRIF, through activation of phosphatase PTPN6 (SHP-1). Related references: PMID:22043923
• The protein is localized on the cell membrane as a single-pass type I membrane protein.
• CD300a is expressed broadly on immune cells including NK cells, T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes, and monocytes.
• Post-translational modifications include phosphorylation on tyrosine residues (important for ITIM-based signaling), glycosylation, and disulfide bond formation.
• Alternative splicing produces multiple isoforms, likely contributing to the observed protein weight variants (33kDa full-length, plus 20kDa, 12kDa, 16.5kDa bands).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 18-178), so this antibody clone should recognize surface CD300a on live cells, suitable for flow cytometry.
• This product is pre-conjugated to dye; ensure flow cytometer configuration includes an appropriate laser (e.g., 561 nm or 594 nm) and detector for PE/DsRed/mCherry-like emission (max ~617 nm). Validate against appropriate isotype and positive/negative control cells.
• For potential western blotting, note that the protein weight variants listed may reflect isoforms or cleavage products; optimize denaturing and reducing conditions and validate in your sample system.
• No cross-reactivity data with other species or family members is provided; verify specificity if using in non-human contexts or among CD300 family proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides researchers and diagnostic manufacturers with streamlined access to IVD raw materials and technical expertise. This anti-CD300a monoclonal antibody supports studies in immunology, oncology, and immune checkpoint research. Beyond individual products, we offer customized sourcing solutions, including validated antibody pairs, bulk reagents, and rare target material procurement to accelerate your assay development.