Rabbit anti-Human CD30 Monoclonal Antibody for Flow Cytometry - P28908

Core Product Specifications and Parameters

Biological Background: CD30 (TNFRSF8) Function and Localization

• CD30 (TNFRSF8, Ki-1) acts as a receptor for CD30 ligand (TNFSF8), playing a role in the regulation of cellular growth and transformation of activated lymphoblasts. Related references: PMID:8391931
• CD30 signaling activates the NF-κB pathway, modulating gene expression and immune responses. Related references: PMID:8999898
• Structurally, CD30 is a transmembrane glycoprotein characterized by disulfide bonds, phosphorylation sites, and extracellular cysteine-rich repeat domains.
• Subcellularly, CD30 localizes predominantly to the cell membrane, with additional cytoplasmic presence.
• At the protein level, CD30 expression has been detected in human alveolar macrophages.
• Alternative initiation and splicing generate multiple isoforms, resulting in observed molecular weights of approximately 14, 50, and 63 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 19-379), which should facilitate recognition of native cell-surface CD30.
• This conjugate enables direct one-step flow cytometry staining; no secondary antibody is required.
• Consider validating the antibody in relevant sample systems (e.g., CD30-positive lymphoma cell lines or activated lymphocytes) under your experimental conditions.
• To maintain conjugate stability, store as recommended and minimize freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering this anti-CD30 monoclonal antibody alongside a comprehensive portfolio of validated antibody pairs, optimized clones, and bulk ancillary reagents. Whether you're developing lymphoma or immune-cell assays, we provide streamlined access to high-quality raw materials to accelerate your diagnostic workflow. Our technical consulting capabilities support you from concept to clinic, ensuring you get the right tools for your research and development needs.