Rabbit anti-Human CD30 Monoclonal Antibody for FC - P28908

Core Product Specifications and Parameters

Biological Background: CD30 Function and Localization

• CD30, also known as Ki-1 antigen and TNFR superfamily member 8 (TNFRSF8), is a receptor for the ligand TNFSF8/CD30L (PMID:8391931).
  Related references:
  PMID:8391931
• The protein is characterized by a signal peptide, extracellular cysteine-rich repeats, a transmembrane helix, and a cytoplasmic domain, with alternative initiation and splicing producing isoforms of varying sizes (e.g., ~14kDa, 50kDa, 63kDa).
• CD30 is primarily expressed on the surface of activated lymphocytes and has been detected in alveolar macrophages, indicating a role in immune cell activation.
• Upon ligand binding, CD30 triggers intracellular signaling cascades that activate NF-kappa-B, thereby regulating gene expression involved in cell growth and transformation (PMID:8999898).
  Related references:
  PMID:8999898
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which are crucial for proper folding and function.
• Subcellularly, CD30 localizes to the cell membrane and can also be found in the cytoplasm, consistent with its receptor and signaling roles.

Experimental Guidance and Technical Tips

• The immunogen (aa 19-379) encompasses the extracellular domain of CD30, making this antibody potentially suitable for detecting surface-expressed CD30 by flow cytometry.
• For flow cytometry applications, consider titrating the antibody to determine the optimal working dilution and include appropriate isotype controls to confirm specificity.
• Validate the antibody in your specific sample matrix (e.g., activated human PBMCs, cell lines expressing CD30) to account for differences in target expression levels and potential matrix effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to support your CD30-related diagnostic and research projects. As a one-stop sourcing partner, we streamline procurement from development to commercialization, ensuring you have the tools needed to advance lymphocyte activation and immuno-oncology studies. Contact us to explore how our CD30 monoclonal antibody and custom raw material solutions can accelerate your workflow.