Rabbit anti-Human CD298/ATP1B3 Monoclonal Antibody for FC - P54709

Core Product Specifications and Parameters

Biological Background: CD298/ATP1B3 Function and Localization

• CD298/ATP1B3 is the beta-3 subunit of the Na+/K+-transporting ATPase (sodium/potassium pump), a non-catalytic component that assembles with the catalytic alpha subunit to form the active enzyme complex.
• The Na+/K+-ATPase catalyzes ATP hydrolysis to drive the coupled exchange of intracellular Na+ for extracellular K+ across the plasma membrane, essential for maintaining electrochemical gradients.
• Alternative names include sodium/potassium-dependent ATPase subunit beta-3 and ATPB-3; the gene is designated ATP1B3.
• While the exact role of the beta-3 subunit remains incompletely understood, it is thought to contribute to enzyme maturation, membrane targeting, and stability.
• CD298 localizes predominantly to the basolateral membrane in polarized epithelial cells, with additional localizations to the apical membrane and melanosomes.
• Key post-translational modifications include disulfide bonds and glycosylation, consistent with its trafficking through the secretory pathway; the protein is a type II transmembrane protein with a signal-anchor sequence.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to ; protect from light and titrate to determine optimal working concentration for flow cytometry.
• As a rabbit monoclonal antibody, it offers consistent lot-to-lot performance; store at 2–8°C upon receipt and avoid repeated freeze-thaw cycles.
• CD298 is a transmembrane protein with a large extracellular domain (aa 66–279) targeted by the recombinant immunogen; it is expected to detect cell-surface expression in live, non-permeabilized cells.
• Include appropriate isotype controls and single-color compensation controls in multicolor flow cytometry panels.
• Validate antibody reactivity in the relevant human sample type (e.g., cell lines, primary cells) given reported expression in melanosomes and epithelial membranes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with one-stop access to high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This CD298/ATP1B3 antibody supports flow cytometry research into ion transport and membrane protein biology. For bulk or customized sourcing needs in the sodium/potassium ATPase field, our team is ready to assist.