Rabbit anti-Human CD298/ATP1B3 mAb for FC - P54709

Core Product Specifications and Parameters

Biological Background: Sodium/potassium-transporting ATPase subunit beta-3 (CD298/ATP1B3) Function and Localization

• The protein encoded by the ATP1B3 gene is the beta-3 subunit of the Na+/K+-ATPase, a heterodimeric enzyme consisting of catalytic alpha subunits and regulatory beta subunits.
• This subunit acts as the non-catalytic component of the active enzyme, which hydrolyzes ATP to drive the coupled exchange of Na+ and K+ ions across the plasma membrane; however, the precise function of the beta-3 subunit remains unclear.
• ATP1B3 is also known as CD298, a cell surface antigen, and ATPB-3.
• It localizes primarily to the apical and basolateral plasma membrane, and has additionally been detected in melanosomes, reflecting its role in ion homeostasis at the cell periphery.
• The protein is a type II membrane glycoprotein featuring a signal-anchor, a single transmembrane helix, and conserved disulfide bonds.
• Alternative splicing gives rise to multiple isoforms, and post-translational modifications include N-linked glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein spanning extracellular amino acids 66–279, which is predicted to encompass the large extracellular domain; this design may favor detection of surface-expressed CD298/ATP1B3.
• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your cell type, and include an appropriate -conjugated isotype control.
• The direct conjugate eliminates the need for a secondary detection step, reducing non-specific binding and simplifying the staining protocol.
• Because CD298/ATP1B3 is a transmembrane protein with an extracellular domain, standard surface staining protocols without permeabilization should suffice; validate expression in your cell system of interest using relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. For ion transport and membrane protein targets such as ATP1B3, we offer one-stop access to bulk ancillary reagents and rare-target sourcing to support your assay development pipeline. Explore our catalog to streamline procurement from concept to clinic.