Rabbit anti-Human CD273/PD-L2 Monoclonal Antibody for Flow Cytometry - Q9BQ51

Core Product Specifications and Parameters

Biological Background: CD273/PD-L2 Function and Localization

• PD-L2 (Programmed cell death 1 ligand 2), also known as B7-DC and CD273, is a member of the B7 family of immune regulatory proteins.
• It plays a critical role in induction and maintenance of immune tolerance to self.
• PD-L2 functions as a ligand for the inhibitory receptor PD-1 (PDCD1), inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production. Related references: PMID:11224527 PMID:12538684
• The protein localizes to the cell membrane and endomembrane system, and can also be secreted.
• Tissue expression is broad, with high levels in heart, placenta, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus.
• As a glycoprotein with immunoglobulin-like domains, PD-L2 contains disulfide bonds and a transmembrane helix, characteristic of immune checkpoint molecules.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20-219) of human PD-L2, which may allow the antibody to detect native surface-expressed PD-L2 in flow cytometry. Validate reactivity in the relevant human cell or tissue sample system.
• For optimal performance in flow cytometry, consider titrating the antibody and using appropriate isotype controls. Ensure proper compensation settings when using dye.
• PD-L2 is expressed on antigen-presenting cells; confirm expression in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support to accelerate diagnostic development. For immune checkpoint research, we provide validated antibody pairs, monoclonal antibodies like this anti-PD-L2 clone, and bulk ancillary reagents. Contact us to seamlessly fulfill your sourcing needs from concept to clinic.