Rabbit anti-Human CD273/PD-L2 mAb for Flow Cytometry - Q9BQ51

Core Product Specifications and Parameters

Biological Background: PD-L2 (CD273) Function and Localization

• PD-L2 (CD273, B7-DC) is a type I transmembrane glycoprotein and member of the B7 family of immune regulatory ligands.
• It negatively regulates T-cell responses by binding to the PD-1 receptor, leading to suppression of T-cell proliferation, cell cycle arrest, and reduced cytokine production (PubMed:11224527). Related references: PMID:11224527
• PD-L2 is critically involved in the induction and maintenance of immune tolerance to self, thereby preventing autoimmunity (PubMed:11224527, PubMed:12538684). Related references: PMID:11224527, PMID:12538684
• Its protein structure includes an extracellular IgV-like domain, a transmembrane helix, and a short cytoplasmic tail; alternative splicing produces both membrane-anchored and soluble secreted isoforms.
• Subcellularly, PD-L2 is found on the cell surface, within the endomembrane system, and as a secreted protein, consistent with its role in both juxtacrine and paracrine signaling.
• It is highly expressed in non-lymphoid tissues like heart, placenta, pancreas, lung, and liver, with lower expression in lymphoid organs (spleen, lymph nodes, thymus), suggesting a role in peripheral tolerance.
• PD-L2 signaling is essential for maintaining immune homeostasis and preventing autoimmune responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (a.a. 20-219), encompassing the IgV-like domain critical for PD-1 binding. This design may favor detection of the native receptor-binding conformation in flow cytometry.
• For FC applications, consider titrating the antibody and using appropriate isotype controls to assess background staining, as PD-L2 expression levels can vary among different cell types.
• Due to the existence of a secreted form, cell surface staining might be affected by competitive shedding or internalization; validate in the relevant sample system.
• PD-L2 expression can be induced by inflammatory cytokines; thus, stimulation conditions may influence detection.
• This antibody is specific for human PD-L2; cross-species reactivity should be verified if used in non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD antibodies, reagents, and technical support—from early-stage development to clinical applications. This anti-PD-L2 monoclonal antibody supports immune checkpoint research and flow cytometry-based diagnostics development. Leverage CamelBio’s portfolio of validated antibody pairs, bulk reagents, and sourcing capabilities for rare targets to accelerate your biomarker discovery and assay development.