Rabbit Anti-Human CD270/HVEM Monoclonal Antibody for Flow Cytometry - Q92956

Core Product Specifications and Parameters

Biological Background: CD270/HVEM Function and Localization

• HVEM (TNFRSF14, CD270, ATAR, TR2) is a glycosylated, disulfide-bonded tumor necrosis factor receptor superfamily member that undergoes alternative splicing, producing multiple isoforms.
• It is localized to the cell membrane as a single-pass transmembrane protein.
• Widely expressed, with highest levels in lung, spleen, and thymus; present on a subpopulation of B cells, monocytes, and naive T cells. Related references: PMID:18193050, PMID:19915044
• HVEM serves as a receptor for four distinct ligands: the TNF superfamily members TNFSF14/LIGHT and lymphotoxin-α, and the immunoglobulin superfamily members BTLA and CD160, integrating stimulatory and inhibitory signals. Related references: PMID:10754304, PMID:18193050, PMID:23761635
• Ligation by LIGHT triggers the TRAF2-TRAF3 E3 ligase pathway, delivering costimulatory signals that promote T cell survival, proliferation, and effector functions. Related references: PMID:10754304, PMID:19915044, PMID:9153189, PMID:9162022
• On NK cells, interaction with CD160 enhances interferon-γ production and anti-tumor immune responses; on activated CD4+ T cells, it downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immunity. Related references: PMID:23761635, PMID:18193050
• Cis-association with BTLA on the same cell maintains naive T cell quiescence, while trans-interactions during adaptive responses provide survival signals to effector T cells. Related references: PMID:19915044
• Additionally, HVEM functions as a cellular receptor for herpes simplex virus 1 and 2, mediating viral entry.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 39–162) of human CD270/HVEM; thus, the antibody likely recognizes an epitope in this region. Consider that it may detect both membrane-bound and soluble receptor forms.
• conjugate enables direct flow cytometry; titrate the antibody to establish the optimal staining index for your cell panel.
• HVEM is expressed on B cells, T cells, and monocytes; include appropriate isotype controls, lineage markers, and Fc receptor blockade to confirm specificity.
• Due to multiple ligand interactions, pre-incubation with recombinant LIGHT, BTLA, or CD160 may be used to assess competitive binding in receptor occupancy experiments.
• For functional assays, note that HVEM signaling can be bidirectional; validate the antibody’s effects under your specific stimulation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CD270/HVEM antibody supports immunology and immuno-oncology research, such as immune checkpoint profiling, viral entry mechanism studies, and lymphocyte activation analyses. Whether you require bulk conjugated antibodies, validated antibody pairs, or custom raw-material sourcing, CamelBio streamlines your procurement to accelerate diagnostic development.