Rabbit anti-Human CD27 Monoclonal Antibody for Flow Cytometry - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (TNFRSF7, T14) is a costimulatory immune-checkpoint receptor expressed on the surface of T-cells, NK-cells, and B-cells, activated by its ligand CD70 (PubMed:28011863). Related references: PMID:28011863
• The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion, playing a critical role in immune surveillance of B-cells (PubMed:28011863). Related references: PMID:28011863
• Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis, which inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed:38354704). Related references: PMID:38354704
• CD27 activation on early progenitor cells provides a negative feedback signal to leukocyte differentiation, thus modulating hematopoiesis (By similarity).
• CD27 negatively regulates the function of Th2 lymphocytes in adipose tissue (By similarity).
• CD27 is a transmembrane receptor located at the cell membrane.
• It is found in most T-lymphocytes.
• Keywords: CD27 is a glycoprotein and phosphoprotein involved in apoptosis, containing disulfide bonds and a signal peptide.

Experimental Guidance and Technical Tips

• This -conjugated rabbit monoclonal antibody is designed for flow cytometry (FC). Ensure your instrument is configured for detection (similar to Alexa Fluor 594).
• The immunogen corresponds to the extracellular domain (amino acids 21-192) of human CD27, ensuring recognition of the native receptor on live cells.
• Validate antibody performance in your specific sample system, particularly with T-lymphocytes where CD27 is highly expressed.
• Consider using appropriate isotype controls and Fc receptor blocking to minimize background.
• For multi-color panels, check fluorophore compatibility and compensation settings.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and recombinant proteins. This anti-CD27 antibody supports immuno-oncology and immune profiling research, and we offer bulk ancillary reagents and customized sourcing for rare targets. Partner with us to streamline your assay development from concept to clinic.