Rabbit Anti-Human CD261/TRAIL-R1 Monoclonal Antibody for Flow Cytometry - O00220

Core Product Specifications and Parameters

Biological Background: CD261/TRAIL-R1 Function and Localization

• CD261, also known as TRAIL-R1, DR4, or TNFRSF10A, is a death receptor belonging to the tumor necrosis factor receptor superfamily that specifically binds the cytotoxic ligand TNFSF10/TRAIL to induce apoptosis (PubMed:26457518, PubMed:38532423). Related references: PMID:26457518, PMID:38532423
• Upon TRAIL engagement, the receptor oligomerizes and recruits the adaptor protein FADD, which then recruits procaspase-8 to form the death-inducing signaling complex (DISC). This triggers autoproteolytic activation of caspase-8 and initiates the caspase cascade leading to apoptosis (PubMed:19090789). Related references: PMID:19090789
• In addition to apoptosis, TRAIL-R1 signaling can promote activation of the NF-kappa-B pathway, contributing to transcriptional regulation of immune, survival, and inflammation-associated genes (PubMed:9430227). Related references: PMID:9430227
• Expression is widespread, with highest levels observed in spleen, peripheral blood leukocytes, small intestine, thymus, and certain tumor cell lines including K-562 and MCF-7; it is also expressed on activated T-cells.
• Subcellularly, TRAIL-R1 is predominantly found on the cell membrane and within membrane rafts, though it can also localize to the cytoplasm and cytosol.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, which are characteristic of its function as a transmembrane signaling receptor.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , making it suitable for direct flow cytometry without the need for secondary detection reagents.
• The immunogen corresponds to the extracellular domain (amino acids 124-232), suggesting the antibody likely recognizes the native surface receptor; validate reactivity in appropriate cell lines expressing CD261.
• Optimize staining concentrations by titration using known positive and negative control cells (e.g., K-562 or MCF-7 for positive, receptor-negative cells for negative).
• For intracellular detection, consider using appropriate permeabilization methods, though the primary application is surface staining.
• Avoid prolonged light exposure to maintain fluorophore stability and minimize photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research institutes, and clinical labs by providing a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, antibody pairs, and ancillary reagents. For researchers investigating TRAIL-induced apoptosis and immune signaling, CamelBio offers expert sourcing for CD261/TRAIL-R1 reagents and related pathway targets, ensuring seamless transition from research to diagnostic development. Our supply capabilities extend to bulk quantities, custom formulations, and hard-to-find targets, helping you streamline your entire workflow.