Rabbit anti-Human CD261/TRAIL-R1 Monoclonal Antibody for Flow Cytometry - O00220

Core Product Specifications and Parameters

Biological Background: TRAIL-R1 (CD261/DR4) Function and Localization

• TRAIL-R1 (CD261/DR4) is a receptor for the cytotoxic ligand TNFSF10/TRAIL. Upon binding, it recruits the adaptor FADD and caspase-8, forming the death-inducing signaling complex (DISC) to initiate apoptosis. Related references: PMID:26457518 PMID:38532423 PMID:19090789
• Promotes the activation of NF-kappa-B, linking TRAIL signaling to inflammatory and survival pathways. Related references: PMID:9430227
• A transmembrane receptor containing an extracellular cysteine-rich domain and a cytoplasmic death domain; primarily located on the cell membrane and membrane rafts, but also detected in the cytosol.
• Widely expressed, with high levels in spleen, peripheral blood leukocytes, small intestine, thymus, and certain cancer lines such as K-562 and MCF-7 cells.
• The immunogen for this antibody spans amino acids 124–232 of the extracellular domain, encompassing the TRAIL-binding region, ensuring target specificity.
• Post‑translational modifications include disulfide bond formation, glycosylation, phosphorylation, and methylation, which may regulate receptor function and trafficking.
• Member of the tumor necrosis factor receptor superfamily; shares structural and functional homology with the decoy receptors TRAIL-R3 and TRAIL-R4.

Experimental Guidance and Technical Tips

• The conjugate allows direct detection in flow cytometry without the need for secondary antibodies; titrate the antibody to optimize signal-to-noise ratio in your specific assay.
• Given the cell surface expression of TRAIL-R1, live-cell staining is recommended. If intracellular pool detection is required, consider fixation and permeabilization steps, though the epitope maps to the extracellular domain.
• Include appropriate isotype controls and untreated cell controls to distinguish specific staining from background.
• Validate antibody performance in TRAIL-R1‑positive cell lines (e.g., K-562, MCF-7) under standardized staining conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research laboratories, and biotech developers with a comprehensive sourcing gateway for IVD raw materials. This TRAIL-R1 antibody exemplifies our commitment to supplying optimized monoclonal antibodies and critical research reagents for apoptosis and oncology studies. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and specialized sourcing services for rare or custom targets—streamlining your path from concept to klinical application.