Rabbit anti-Human CD244/2B4 Monoclonal Antibody for Flow Cytometry - Q9BZW8

Core Product Specifications and Parameters

Biological Background: CD244/2B4 Function and Localization

• CD244, also known as 2B4, is a natural killer cell receptor belonging to the signaling lymphocytic activation molecule (SLAM) family. It functions as a heterophilic receptor with CD48 as its ligand, modulating immune cell activation and differentiation.
• Acts as an activating NK cell receptor upon engagement with SH2D1A/SAP and FYN, leading to downstream signaling through VAV1, INPP5D/SHIP1, and CBL. This triggers NK cell cytotoxicity, IFN-γ production, and granule exocytosis (PubMed:10359122, PubMed:11714776, PubMed:8376943, PubMed:15713798).
  Related references:
  PMID:10359122 PMID:11714776 PMID:8376943 PMID:15713798
• In the absence of SH2D1A, CD244 can transduce inhibitory signals mediated by INPP5D, PTPN6/SHP-1, and PTPN11/SHP-2, ensuring self-tolerance during early NK cell development (PubMed:10934222, PubMed:11917118).
  Related references:
  PMID:10934222 PMID:11917118
• CD244 enhances NK cell activation by costimulating signals from other receptors like NCR3 and NCR1 (PubMed:10741393).
  Related references:
  PMID:10741393
• Beyond NK cells, CD244 is expressed on CD8+ T-cells, monocytes, basophils, and dendritic cells, where it regulates T-cell proliferation and dampens inflammatory responses (By similarity).
• The protein localizes to the cell membrane and is enriched in membrane rafts, facilitating signal transduction. It undergoes alternative splicing, yielding isoforms with predicted molecular weights of 30, 36, and 41 kDa.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, which are critical for its function and surface expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-221) of human CD244, covering the Ig-like regions. This design targets the receptor's ligand-binding interface, making the antibody suitable for blocking or detecting surface CD244 in flow cytometry applications.
• For FC, titrate the antibody to identify the optimal staining concentration in your cell system. Include appropriate isotype controls and consider using Fc receptor blocking reagents to minimize non-specific binding.
• Since the product is validated for flow cytometry, cross-reactivity with other applications (e.g., Western blot, IHC) is not guaranteed. Validate in the relevant sample system if considering alternative uses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. With expertise in immune receptor targets like CD244, we also offer rare target raw-material sourcing to streamline your development from discovery to clinical application. Partner with us to access reliable, cost-effective solutions for your NK cell and immune profiling needs.