Rabbit anti-Human CD24 Monoclonal Antibody for FC - P25063

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24 is a signal transducer protein, also known as small cell lung carcinoma cluster 4 antigen.
• It plays a pivotal role in the differentiation of various cell types; signaling is triggered by lectin-like ligand binding to its carbohydrates and transduced via GPI-anchor-derived second messengers.
• CD24 modulates B-cell activation responses: it promotes antigen-dependent proliferation of B-cells and prevents their terminal differentiation into antibody-forming cells. Related references: PMID:11313396
• In association with SIGLEC10, CD24 may be involved in the selective suppression of immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90.
• It plays a role in the control of autoimmunity (by similarity).
• CD24 is localized at the cell membrane and attached via a GPI-anchor.
• Expressed predominantly on B-cells, but also found on T-cells and various cell lines including erythroleukemia and small cell lung carcinoma lines.
• The protein undergoes alternative splicing and glycosylation, with a molecular weight of approximately 8 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-80) of human CD24, targeting the extracellular domain accessible for live cell staining in flow cytometry.
• For FC applications, titrate the antibody to determine optimal working concentration and consider using relevant isotype controls.
• CD24 is a GPI-anchored surface protein; avoid permeabilization unless intracellular staining is intended, as it may affect surface epitope accessibility.
• Validate staining in the sample system of interest, as expression may vary across different cell types and conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. Whether you need anti-CD24 antibodies for B-cell immunology research or rare target raw-material sourcing, CamelBio provides tailored supply support to accelerate your project from concept to clinic.