Rabbit anti-Human CD24 mAb for IF/ICC, FC - P25063

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24 (Signal transducer CD24) is a small, heavily glycosylated GPI-anchored cell surface protein, also known as the small cell lung carcinoma cluster 4 antigen.
• It plays a role in cell differentiation and modulates B-cell activation responses.
• CD24 promotes antigen-dependent proliferation of B-cells and prevents terminal differentiation into antibody-forming cells. Related references: PMID:11313396
• In association with SIGLEC10, CD24 may selectively suppress immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1 and heat shock proteins.
• Subcellular localization is primarily at the cell membrane, consistent with its GPI-anchor linkage.
• Expressed on B-cells and also on the surface of T-cells, as well as various hematopoietic and carcinoma cell lines.
• Keywords include Alternative splicing, Glycoprotein, GPI-anchor, and Signal, reflecting its structural features and post-translational modifications.
• Protein molecular weight is approximately 8-13 kDa depending on glycosylation and isoform (consistent with UniProt 8.1 kDa and observed 13 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 1-80), suggesting the antibody recognizes an epitope accessible on the cell surface without permeabilization.
• For flow cytometry, consider using live, unfixed cells to detect surface CD24 expression; direct conjugate minimizes secondary antibody steps.
• For immunofluorescence, validate staining in human B-cell lines (e.g., Namalwa) as positive controls, and ensure appropriate isotype controls.
• Optimize antibody dilution and incubation conditions depending on the sample and detection system; typical starting concentrations for flow cytometry are 0.5-5 μg/mL.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a streamlined supply of high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-human CD24 antibody supports immunology and oncology research applications, and CamelBio ensures reliable access to such specialized tools along with technical consultation to accelerate your assay development and production scaling.