Rabbit anti-Human CD239/BCAM Monoclonal Antibody for Flow Cytometry - P50895

Core Product Specifications and Parameters

Biological Background: BCAM Function and Localization

• BCAM is a transmembrane glycoprotein that functions as both a receptor and adhesion molecule, playing a crucial role in cell adhesion, motility, migration, and invasion. Related references: PMID:9616226, PMID:31413112
• The extracellular domain of BCAM binds to extracellular matrix proteins such as laminin and integrin, while its intracellular domain interacts with cytoskeletal proteins like hemoglobin to facilitate signal transduction. Related references: PMID:17158232
• It acts as a receptor for laminin alpha-5 (LAMA5), promoting cell adhesion. Related references: PMID:15975931
• JAK2-mediated phosphorylation activates BCAM adhesion to laminin through a Rap1/AKT signaling pathway, particularly in the absence of EPOR. Related references: PMID:23160466
• BCAM, also known as Lutheran blood group glycoprotein and Auberger B antigen, is a cell membrane protein belonging to the immunoglobulin superfamily.
• It is widely expressed, with highest levels in the pancreas and very low in the brain, and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 32-547) of human CD239/BCAM, suggesting the antibody recognizes an N-terminal epitope suitable for detecting cell surface expression by flow cytometry.
• As a directly conjugated antibody, it can be used for single-step flow cytometry staining without a secondary antibody.
• For live-cell staining, a standard staining buffer without detergent is recommended, as BCAM is a transmembrane protein with extracellular exposure.
• Consider titrating the antibody to determine optimal concentration for your specific cell type and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD239/BCAM antibody supports research into cell adhesion, migration, and blood group antigen biology, enabling assay development in oncology and transfusion medicine. Contact us for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.