Rabbit anti-Human CD235a/Glycophorin A Monoclonal Antibody for FC - P02724

Core Product Specifications and Parameters

Biological Background: Glycophorin A Function and Localization

• Major intrinsic membrane protein of erythrocytes; component of the ankyrin-1 complex, critical for maintaining erythrocyte membrane stability and shape.
  Related references:
  PMID:35835865
• Carries MN blood group antigens on its extracellular N-terminal domain, determined by specific amino acid polymorphisms and glycosylation patterns.
• Interacts with SLC4A1 (band 3 anion exchanger), facilitating its translocation to the plasma membrane and enhancing its transport activity.
• Acts as a receptor for hepatitis A virus (HAV) and serves as the erythrocyte-binding antigen-175 (EBA-175) receptor for Plasmodium falciparum invasion, with binding dependent on sialic acid residues of O-linked glycans.
• Localized to the cell membrane; a single-pass type I transmembrane glycoprotein with a heavily glycosylated extracellular domain.
• Undergoes alternative splicing and is phosphorylated; identified as a sialic acid-rich glycoprotein.
• Broadly classified under keywords: Blood group antigen, Host cell receptor for virus entry, Host-virus interaction, Cell membrane, Glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 20–91) covers the extracellular N-terminal region containing MN blood group epitopes; the antibody is expected to recognize the extracellular domain.
• Designed for flow cytometry (FC); ensure appropriate red blood cell gating and avoid excessive fixation that may mask epitopes.
• Validate antibody specificity using GYPA-negative cell lines or siRNA knockdown in erythroid progenitor models if available.
• Titrate the antibody to determine optimal staining concentration for your specific flow cytometry protocol.

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CamelBio is your integrated partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether you are developing hematology assays or researching erythrocyte membrane biology, CamelBio bridges your concept to clinic with reliable supply solutions tailored to diagnostic manufacturers and research laboratories.