Rabbit anti-Human CD235a/Glycophorin A Monoclonal Antibody for FC - P02724

Core Product Specifications and Parameters

Biological Background: Glycophorin-A Function and Localization

• Glycophorin-A (GYPA), also known as MN sialoglycoprotein, PAS-2, or CD235a, is the major sialoglycoprotein of the human erythrocyte membrane.
• It is a critical component of the ankyrin-1 complex, which maintains erythrocyte membrane stability and shape. Related references: PMID:35835865
• The extensively glycosylated N‑terminal extracellular domain carries the MN blood group antigens and mediates interactions with pathogens: it serves as a receptor for hepatitis A virus (HAV) and for P. falciparum erythrocyte‑binding antigen‑175 (EBA‑175) via sialic acid residues.
• GYPA facilitates the translocation and full functional activity of the anion exchanger SLC4A1 (band 3) to the plasma membrane.
• Subcellularly, the protein spans the cell membrane with a single transmembrane helix; alternative splicing generates multiple isoforms.
• Post‑translational modifications include extensive O‑linked glycosylation rich in sialic acids, and phosphorylation, consistent with its classification as a phosphoprotein and glycoprotein.
• Relevant UniProt keywords: Blood group antigen, Host cell receptor for virus entry, Glycoprotein, Transmembrane, Sialic acid, and Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20‑91 of the extracellular domain (NP_002090.4), encompassing the MN blood group antigen region. For flow cytometry, this domain is exposed on live, non‑permeabilized cells.
• Use appropriate isotype controls and single‑stain compensation controls when incorporating the conjugate into multi‑color panels.
• Because pathogen interactions (e.g., P. falciparum EBA‑175 binding) are sialic acid‑dependent, consider relevant enzymatic treatments or blocking steps if studying such interactions.
• Validate reactivity in your specific experimental system, as cross‑reactivity has been confirmed only for human samples; alternative splicing may affect epitope availability in certain cell types.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers one‑stop access to validated IVD raw materials, including this ‑conjugated anti‑CD235a monoclonal antibody for flow cytometry applications. Beyond this product, we supply optimized antibody pairs, bulk ancillary reagents, and rare‑target sourcing to accelerate your assay development from concept to clinic.