Rabbit anti-Human CD235a/Glycophorin A Monoclonal Antibody for FC - P02724

Core Product Specifications and Parameters

Biological Background: Glycophorin-A Function and Localization

• Glycophorin-A is the major intrinsic membrane protein of erythrocytes, with its N-terminal glycosylated segment carrying MN blood group receptors.
• Component of the ankyrin-1 complex, crucial for erythrocyte membrane stability and shape; required for high activity of SLC4A1 and its translocation to the plasma membrane. Related references: PMID:35835865
• Serves as a receptor for Hepatitis A virus (HAV) and for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding is dependent on sialic acid residues of O-linked glycans.
• Localizes to the cell membrane; undergoes alternative splicing and phosphorylation.
• Keywords: blood group antigen, host-virus interaction, sialoglycoprotein, transmembrane, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal extracellular domain (amino acids 20-91), so the antibody is likely to recognize the extracellular region of Glycophorin-A, suitable for flow cytometry on live or fixed erythrocytes.
• For FC, consider titrating the antibody on human red blood cells or erythroleukemia cell lines (e.g., K562) to determine optimal staining concentration.
• Validate specificity by using Glycophorin A‑knockout cell lines or blocking peptides in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for erythrocyte biology and infectious disease research. Whether you need this Glycophorin A antibody for flow‑based blood group antigen assays or other custom solutions, we streamline procurement from concept to clinic.