Rabbit anti-Human CD228/MFI2 Monoclonal Antibody for Flow Cytometry - P08582

Core Product Specifications and Parameters

Biological Background: CD228/MFI2 (Melanotransferrin) Function and Localization

• Melanotransferrin (CD228/MFI2) is a cell membrane glycoprotein involved in iron cellular uptake; it binds one iron atom per subunit and may also bind zinc.
• The protein is internalized and then recycled back to the cell membrane, suggesting a role in iron transport and homeostasis.
• It is predominantly expressed in human melanomas, certain fetal tissues, and also found in liver, epithelium, umbilical cord, placenta, and sweat gland ducts.
• Encoded by the MELTF gene (MAP97, MFI2), alternative splicing may produce isoforms; the full-length protein has a molecular weight of approximately 80 kDa (UniProt: 80,215 Da), with a possible processed form at ~32 kDa.
• Subcellular localization is primarily at the cell membrane, and the protein is attached via a GPI-anchor, classifying it as a lipid-anchored membrane protein.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, consistent with its cell surface role.
• It belongs to the transferrin family and has been studied in melanoma biology, making it a potential target for cancer research.

Experimental Guidance and Technical Tips

• For flow cytometry, use an appropriate cell surface staining protocol with live cells; avoid permeabilization to preserve membrane localization of CD228.
• Validate antibody binding in melanoma cell lines known to express CD228 (e.g., SK-MEL-28) to ensure specificity.
• Include isotype controls and Fc receptor blocking steps to minimize nonspecific binding.
• Optimize detector settings for the fluorophore (excitation/emission similar to PE-Texas Red) on your flow cytometer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical support to accelerate your diagnostic development. This anti-CD228/MFI2 antibody is part of our extensive catalog of validated monoclonal antibodies for flow cytometry applications. We also provide customized sourcing for rare targets, bulk ancillary reagents, and optimized antibody pairs to meet your assay requirements — all through a single, streamlined partner.