Rabbit anti-Human CD228/MFI2 Monoclonal Antibody for Flow Cytometry - P08582

Core Product Specifications and Parameters

Biological Background: CD228/MFI2 Function and Localization

• Encoded by the MELTF gene (also known as MAP97, MFI2), melanotransferrin is a cell-surface glycoprotein of the transferrin family. Alternative splicing yields isoforms, with the full-length protein at ~80 kDa and a secreted isoform at ~32 kDa.
• Functions in cellular iron uptake: binds one ferric ion per subunit, internalizes, and recycles back to the plasma membrane; may also transport zinc.
• Originally identified as melanoma-associated antigen p97, it is predominantly expressed in human melanomas, certain fetal tissues, liver, epithelium, umbilical cord, placenta, and sweat gland ducts.
• Anchored to the cell membrane via a C-terminal GPI moiety, acting as a lipid-anchored transporter rather than a soluble carrier.
• Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, as identified by proteomics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20-708, encompassing nearly the full-length extracellular domain; verify reactivity with native and recombinant CD228 in your flow cytometry panel.
• As a directly conjugated antibody, no secondary reagent is needed; titrate from 0.1–1 µg per million cells to determine optimal staining index.
• Validate specificity using CD228-silenced cells or a known negative cell line; track viability and doublet exclusion to avoid artifacts from dying cells with high autofluorescence.
• GPI-anchored proteins can be sensitive to trypsin; prepare single-cell suspensions with gentle enzymatic or mechanical dissociation if working with adherent cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement from discovery to commercialization. This anti-CD228 monoclonal antibody supports melanoma biomarker and iron metabolism research, and our catalog includes matched antibody pairs, bulk reagents, and custom sourcing for rare targets—helping you move from concept to clinic with confidence.