Rabbit anti-Human CD226/DNAM-1 Monoclonal Antibody for Flow Cytometry - Q15762

Core Product Specifications and Parameters

Biological Background: CD226/DNAM-1 Function and Localization

• CD226, also known as DNAM-1 (DNAX accessory molecule 1), PTA1, or TLiSA1, is a transmembrane glycoprotein receptor expressed on immune cells such as T-lymphocytes and NK cells.
• It functions as a costimulatory receptor that mediates intercellular adhesion, lymphocyte signaling, cytotoxicity, and cytokine secretion in cytotoxic T-cells and NK cells. Related references: PMID:8673704 PMID:9712030
• Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells (e.g., virus-infected or tumor cells), CD226 enhances the cytotoxic activity of NK cells and CTLs. Related references: PMID:26755705 PMID:31253644 PMID:30591568
• Mechanistically, phosphorylation by Src kinases (LYN or FYN) enables recruitment of the adaptor GRB2, leading to activation of VAV1, PI3K, PLCG1, ERK, AKT, and calcium fluxes.
• The protein contains immunoglobulin domains, a transmembrane helix, and disulfide bonds; it is classified as a cell adhesion receptor and phosphoprotein.
• Subcellular localization is at the cell membrane, and tissue specificity is restricted to peripheral blood T-lymphocytes.
• UniProt lists the calculated molecular weight of CD226 as approximately 38.6 kDa for the unmodified protein.

Experimental Guidance and Technical Tips

• This antibody is generated against the extracellular domain (amino acids 19-247) of human CD226 and is recommended for flow cytometry applications.
• As a directly conjugated antibody, ensure proper compensation and use appropriate isotype controls to assess nonspecific binding.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Cross-reactivity with species other than human has not been confirmed; validate in the relevant sample system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing high-quality IVD raw materials, including this anti-CD226/DNAM-1 antibody, which is ideal for immune monitoring and tumor immunity studies. Our portfolio encompasses validated antibody pairs, optimized recombinant monoclonal antibodies, and bulk ancillary reagents, ensuring seamless procurement for assay development. For custom or rare-target needs, CamelBio offers specialized sourcing and technical consulting to accelerate your project from concept to clinic.