Rabbit anti-Human CD226/DNAM-1 mAb - Q15762

Core Product Specifications and Parameters

Biological Background: CD226/DNAM-1 Function and Localization

• CD226 (also known as DNAM-1, PTA1, TLiSA1) is a cell surface receptor belonging to the immunoglobulin superfamily. It plays a critical role in the immune system, mediating intercellular adhesion, lymphocyte signaling, and the cytotoxicity and lymphokine secretion of cytotoxic T-cells and NK cells. Related references: PMID:8673704 PMID:9712030

• Upon recognition of target cells like virus-infected or tumor cells, CD226 acts as a costimulatory receptor. Binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells enhances the cytotoxic activity of NK cells and CTLs, facilitating their killing of these cells. Related references: PMID:26755705 PMID:31253644 PMID:30591568

• Mechanistically, CD226 is phosphorylated by Src family kinases such as LYN or FYN, enabling binding to the adapter GRB2. This interaction leads to activation of VAV1, PI3K, PLCG1, and downstream kinases ERK and AKT, as well as calcium fluxes.

• CD226 is a transmembrane protein located on the cell membrane.

• It is specifically expressed by peripheral blood T-lymphocytes.

• Structurally, CD226 contains immunoglobulin domains, disulfide bonds, and is a glycoprotein. The UniProt canonical sequence has a calculated molecular weight of approximately 38.6 kDa, while the mature glycosylated form migrates at around 39 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• As a monoclonal antibody generated against the extracellular domain (aa 19-247) of human CD226, this antibody is designed for flow cytometry. Consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental conditions.

• Verify expression of CD226 on your target cells (e.g., T-cells, NK cells) using appropriate positive controls. Use isotype control antibodies to assess background staining.

• For multi-color flow cytometry, be aware of spectral overlap when using with other fluorophores. Proper compensation is recommended.

• The antibody is specific for human CD226; cross-reactivity with other species has not been tested. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this CD226/DNAM-1 monoclonal antibody as part of a comprehensive portfolio of IVD raw materials for immuno-oncology and immune profiling. As a one-stop sourcing bridge, we provide diagnostic manufacturers with validated antibody pairs, bulk ancillary reagents, and access to rare target raw materials—streamlining your development from concept to clinic.