Rabbit anti-Human CD226/DNAM-1 mAb for FC - Q15762

Core Product Specifications and Parameters

Biological Background: CD226/DNAM-1 Function and Localization

• CD226 (DNAM-1) is a cell surface receptor belonging to the immunoglobulin superfamily, involved in intercellular adhesion, lymphocyte signaling, and cytotoxicity mediated by cytotoxic T-cells and NK cells. Related references: PMID:8673704, PMID:9712030
• It functions as a costimulatory receptor upon recognition of target cells, binding to its ligands PVR/CD155 or NECTIN2/CD112 on virus-infected or tumor cells, thereby enhancing NK and CTL cytotoxic activity. Related references: PMID:26755705, PMID:31253644, PMID:30591568
• Upon ligand engagement, CD226 is phosphorylated by Src family kinases such as LYN or FYN, then recruits the adapter GRB2, leading to activation of VAV1, PI3K, PLCG1, and downstream kinases ERK and AKT, as well as calcium fluxes.
• CD226 is localized to the cell membrane as a single-pass transmembrane protein.
• It is expressed by peripheral blood T-lymphocytes and plays a role in immune surveillance.
• The protein contains immunoglobulin-like domains, is N-glycosylated, and forms disulfide bonds; its molecular weight is approximately 39 kDa on SDS-PAGE (UniProt: 38.6 kDa).

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the extracellular domain (aa 19-247), this antibody likely recognizes the native receptor on live cells, making it suitable for flow cytometry.
• For FC, consider titrating the antibody to determine optimal staining concentrations; use appropriate isotype controls.
• Due to activation-dependent expression, validate CD226 levels in stimulated versus resting immune cells.
• The antibody cross-reacts with human samples; ensure compatibility with other species if multiplexing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined supply chain for IVD raw materials, including validated antibody pairs, bulk reagents, and technical support. This anti-CD226 monoclonal antibody supports flow cytometry applications in immune-oncology and infectious disease research. Contact us for customized solutions, bulk ancillary reagents, and rare-target sourcing.