Rabbit anti-Human CD22 Monoclonal Antibody for Flow Cytometry - P20273

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22, also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM), is a transmembrane glycoprotein belonging to the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It is highly expressed on B cells and functions as an inhibitory coreceptor that modulates B-cell receptor (BCR) signaling. Related references: PMID:34330755, PMID:8627166
• Upon BCR activation, the immunoreceptor tyrosine-based inhibitory motifs (ITIMs) of CD22 are phosphorylated by the Src kinase LYN, which recruits the protein tyrosine phosphatase PTPN6 (SHP-1). This leads to attenuation of calcium mobilization and cellular activation, negatively regulating B-cell responses and potentially inducing apoptosis under strong signaling. Related references: PMID:8627166, PMID:20516366
• CD22 specifically binds to α2,6-linked sialic acid residues on glycoproteins such as CD45, IgM, and CD22 itself in a cis configuration, and can also serve as an adhesion molecule through trans interactions with ligands on other cells. Related references: PMID:20172905
• The protein plays essential roles in B-cell differentiation, antigen presentation, and trafficking to the bone marrow, with its expression predominantly restricted to mature B lymphocytes. Related references: PMID:34330755, PMID:8627166
• CD22 is subject to alternative splicing, producing variants with different numbers of extracellular Ig-like domains; it is also heavily glycosylated and phosphorylated, features that contribute to its function and regulation.
• The full‑length human CD22 consists of 847 amino acids with a calculated molecular mass of approximately 95.3 kDa, and contains an N‑terminal signal peptide, seven Ig‑like C2‑type domains, a transmembrane helix, and a cytoplasmic tail with ITIM and ITIM-like motifs.

Experimental Guidance and Technical Tips

• The immunogen used (recombinant protein spanning amino acids 20–687) covers the entire extracellular region of human CD22, including all Ig-like domains, which helps ensure recognition of the native protein on live cells.
• As a pre‑conjugated antibody (), this reagent is ready for direct flow cytometry without a secondary staining step; nevertheless, titrate the antibody on relevant positive and negative cell lines to determine the optimal working concentration.
• Common human B‑cell lines (e.g., Raji, Daudi, Ramos) express high levels of CD22 and can serve as positive controls; an isotype control antibody is recommended to assess background binding.
• For multicolor flow cytometry panels, include fluorescence compensation controls and consider that CD22 expression may be modulated by B‑cell activation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and custom solutions—from initial concept through clinical readiness. This CD22 monoclonal antibody, targeting a key B‑cell antigen, is suited for flow cytometry applications in immunology and oncology research. Beyond individual reagents, CamelBio can source validated antibody pairs, bulk ancillary materials, and rare‑target raw materials to accelerate your assay development.