Rabbit anti-Human CD22 Monoclonal Antibody for Flow Cytometry - P20273

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22, also known as Siglec-2, BL-CAM, and Leu-14, is a B-cell receptor belonging to the sialic acid-binding immunoglobulin-like lectin (Siglec) family.
• It is the most highly expressed Siglec on B-cells and plays crucial roles in B-cell differentiation, antigen presentation, and trafficking to the bone marrow. Related references: PMID:34330755 PMID:8627166
• CD22 binds to α2,6-linked sialic acid residues on surface molecules such as CD22 itself, CD45, and IgM in a cis configuration, and can also function as an adhesion molecule by binding ligands on other cells in trans. Related references: PMID:20172905
• It acts as an inhibitory coreceptor on B-cells, where phosphorylation of its immunoreceptor tyrosine-based inhibitory motif (ITIM) by LYN kinase recruits the tyrosine phosphatase PTPN6 (SHP-1), leading to negative regulation of B-cell receptor (BCR) signaling and dampening of calcium mobilization and cellular activation. Related references: PMID:8627166
• Strong negative signaling via CD22 can induce B-cell apoptosis. Related references: PMID:20516366
• The protein is localized at the cell membrane and is specifically expressed on B-lymphocytes.
• It is a glycoprotein with multiple immunoglobulin domains and disulfide bonds, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the entire extracellular domain (amino acids 20–687), making this antibody well-suited for recognizing native CD22 on the surface of live B-cells.
• As a directly conjugated antibody, it eliminates the need for a secondary antibody in flow cytometry, simplifying your staining protocol and reducing non‑specific binding.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions, and include appropriate isotype controls to assess background.
• For best results, ensure cells are viable and well‑gated during flow cytometric analysis. CD22 is highly expressed on B‑cells, so expect strong staining on B‑lymphocyte populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated monoclonal antibodies like this anti‑CD22 clone. Whether you need bulk ancillary reagents, optimized antibody pairs, or help sourcing rare‑target materials for B‑cell immunophenotyping and hematopoietic assay development, our team is ready to support your project from initial concept to clinical application. Partner with CamelBio to streamline your supply chain and accelerate your assay development.