Rabbit anti-Human CD215/IL-15R alpha Monoclonal Antibody for Flow Cytometry - Q13261

Core Product Specifications and Parameters

Biological Background: Interleukin-15 Receptor Subunit Alpha Function and Localization

• IL15RA encodes the alpha subunit of the interleukin-15 receptor, a high-affinity receptor for IL-15 that mediates both cis and trans signaling pathways. Related references: PMID:8530383
• In neutrophils, IL15RA activates the kinase SYK and is required for IL-15-induced phagocytosis. Related references: PMID:15123770
• Multiple protein isoforms are produced by alternative splicing; several isoforms lack the ability to bind IL-15 and may interfere with signal transduction. Related references: PMID:10480910
• The receptor is predominantly localized to the plasma membrane but is also present in intracellular membranes (endoplasmic reticulum, Golgi, cytoplasmic vesicles) and can be secreted.
• Widely expressed, with higher expression in fetal hippocampus and cerebellum; soluble forms are abundant in the brain. Related references: PMID:12114302
• Post-translational modifications include N-linked glycosylation and phosphorylation, which may affect receptor stability and signaling.
• The extracellular region contains a sushi domain (CCP/SCR) critical for ligand binding; the protein is a member of the type I cytokine receptor family.
• Keywords: Alternative splicing, Disulfide bond, Glycoprotein, Phosphoprotein, Sushi.

Experimental Guidance and Technical Tips

• As a far-red conjugate (), optimize laser (e.g., 633–647 nm) and filter (e.g., 660/20 nm or similar) settings for flow cytometry.
• Use an appropriate isotype control (rabbit IgG, same fluorophore) and include Fc receptor blocking for human samples to minimize nonspecific binding.
• The immunogen covers the extracellular domain (aa 31–205), suggesting the antibody recognizes surface-expressed receptor; confirm membrane staining in permeabilization-optional protocols.
• Validate in stimulated versus resting conditions, as IL-15RA surface expression may be modulated by cytokines.
• For multicolor panels, check compensation and consider antigen density; IL-15RA is typically of low to moderate abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-IL-15RA antibody. Whether you need validated antibody pairs, bulk monoclonal/polyclonal antibodies, or ancillary reagents for IL-15/IL-15RA pathway studies, our one-stop service streamlines your supply chain from discovery to clinical application. Contact us for customized sourcing of rare target materials and technical consultation.