Rabbit anti-Human CD215/IL-15R alpha Monoclonal Antibody for Flow Cytometry - Q13261

Core Product Specifications and Parameters

Biological Background: CD215/IL-15R alpha Function and Localization

• CD215/IL-15R alpha, encoded by the IL15RA gene, serves as the high-affinity receptor for interleukin-15 (IL-15), a cytokine critical for immune cell development and function.
  Related references:
  PMID:8530383
• The receptor can signal both in cis and in trans, where membrane-bound IL15RA on one cell presents IL-15 to neighboring cells expressing the IL-2 receptor gamma chain (IL2RG), thereby amplifying immune responses.
• In neutrophils, IL15RA couples IL-15 stimulation to the activation of spleen tyrosine kinase (SYK) and is required for IL-15-induced phagocytosis in a SYK-dependent manner.
  Related references:
  PMID:15123770
• Multiple isoforms arise from alternative splicing; isoforms 1, 3, 4, 5, 6, 7, 8, and 9 are widely expressed, and their expression may modulate or interfere with IL-15 signal transduction.
  Related references:
  PMID:10480910
• The protein localizes to multiple membrane compartments, including the plasma membrane, nuclear membrane, endoplasmic reticulum, Golgi, and cytoplasmic vesicles, and a soluble form (sIL-15RA) can be secreted into the extracellular space.
• In fetal brain, CD215/IL-15R alpha is expressed at higher levels in the hippocampus and cerebellum relative to the cortex and thalamus; the soluble form predominates in all brain structures.
  Related references:
  PMID:12114302
• The protein contains a signal peptide, a sushi domain, and a transmembrane helix; it is a glycoprotein with disulfide bonds and potential phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 31–205) of human CD215/IL-15R alpha, which covers the ligand-binding sushi domain; this design may enable detection of both membrane-anchored and soluble receptor forms in appropriate sample types.
• This antibody is intended for flow cytometry (FC). Titrate the antibody on model human cell lines known to express IL15RA (e.g., neutrophils or peripheral blood mononuclear cells) to optimize staining conditions and minimize background.
• For FC experiments, consider using a viability dye and appropriate isotype controls to ensure specific staining, particularly when analyzing heterogeneous cell populations.
• Because CD215/IL-15R alpha can exist as cell-surface and released soluble forms, fixation and permeabilization protocols may need to be adjusted based on the target compartment of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies, matched pairs, and ancillary reagents. This anti-CD215/IL-15R alpha monoclonal antibody addresses needs in immune receptor and cytokine signaling research, and our team can assist with bulk orders, custom sourcing, and technical consultation to accelerate your assay development—from concept to clinic.