Rabbit anti-Human CD215/IL-15R alpha Monoclonal Antibody for FC - Q13261

Core Product Specifications and Parameters

Biological Background: Interleukin-15 receptor subunit alpha (CD215) Function and Localization

• Functions as the high-affinity receptor for interleukin-15 (IL-15), enabling both cis and trans signaling mechanisms essential for immune cell homeostasis and activation. Related references: PMID:8530383
• In neutrophils, CD215/IL-15Rα binds and activates the kinase SYK upon IL-15 stimulation, and is critically required for IL-15-induced phagocytosis in a SYK-dependent manner. Related references: PMID:15123770
• Multiple isoforms are generated through alternative splicing; some variants lack IL-15 binding ability, and expression of different isoforms can alter or interfere with normal signal transduction. Related references: PMID:10480910
• The receptor localizes predominantly to the plasma membrane but is also found on intracellular membranes including the endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles, and the nucleus, with a secreted soluble form detectable in the extracellular space.
• At the protein level, CD215/IL-15Rα is prominently expressed in neutrophils and shows higher expression in fetal brain structures such as the hippocampus and cerebellum; soluble forms are notably abundant across all brain regions.
• The protein is characterized by structural features including a signal peptide, a sushi domain, a single transmembrane helix, and multiple post-translational modifications such as glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 31–205), making this product suitable for detecting membrane-bound and possibly soluble receptor forms. Validate in the relevant sample system.
• As a directly conjugated antibody, it is ready for flow cytometry without secondary staining. Consider titrating to determine optimal staining concentrations for your cell type.
• Include appropriate isotype controls and live/dead discrimination to ensure specific detection of CD215-expressing cells. Human peripheral blood neutrophils or activated T cells may serve as positive control populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a seamless, one-stop sourcing solution for IVD raw materials, encompassing this monoclonal antibody to the IL-15 receptor alpha (CD215) and the broader immune receptor portfolio. From validated antibody pairs and bulk ancillary reagents to rare target customization, our supply chain is tailored to support your immunoassay and flow cytometry development needs.