Rabbit anti-Human CD213a1/IL-13Rα1 Monoclonal Antibody for FC - P78552

Core Product Specifications and Parameters

Biological Background: IL-13Rα1 Function and Localization

• IL-13Rα1 (CD213a1, CT19) is a low-affinity receptor for interleukin-13 (IL-13).
• It forms a functional IL-13 receptor complex with IL4RA, enabling IL-13 signaling.
• Serves as an alternative accessory protein for IL-4 signaling, substituting for the common gamma chain (IL2RG) in certain contexts.
• Localizes to the cell membrane (predicted transmembrane protein).
• Ubiquitously expressed, with highest levels in heart, liver, skeletal muscle, and ovary; lower in brain, lung, and kidney; also found in B-cells, T-cells, and endothelial cells.
• Contains fibronectin type-III domains and multiple disulfide bonds; glycoprotein; subject to alternative splicing.
• Predicted molecular weight of unmodified protein: ~48.8 kDa; observed bands at 31 kDa and 48 kDa may reflect glycosylation or isoforms.
• Acts as a cancer/testis antigen (CT19) and may be implicated in tumor immunology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 29-343), ensuring recognition of native surface receptor; suitable for flow cytometry (FC) applications.
• conjugation (red fluorophore) is compatible with common flow cytometry instruments; consider appropriate compensation controls.
• Cell surface staining: validate on known IL-13Rα1-expressing cell lines (e.g., immune cells) using standard protocols; use at appropriate antibody dilution determined by titration.
• For intracellular staining, ensure proper permeabilization if targeting intracellular pools.
• Cross-reactivity with human protein confirmed; verify performance in other species if needed.
• Protein weight observations (31 kDa and 48 kDa) may correspond to differentially glycosylated or cleaved forms; confirm band patterns in your model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For IL-13Rα1 research—spanning immunology, oncology, and cytokine signaling—we deliver quality-assured reagents to support your diagnostic and laboratory workflows. Partner with CamelBio to accelerate your project from concept to clinic.