Rabbit anti-Human CD206/MMR Monoclonal Antibody for FC - P22897

Core Product Specifications and Parameters

Biological Background: Macrophage Mannose Receptor 1 Function and Localization

• Macrophage mannose receptor 1 (MMR, also known as CD206, CLEC13D, hMR) is a single-pass type I membrane protein that mediates endocytosis of glycoproteins by binding sulfated and non-sulfated polysaccharide chains on ligands.
• Functions as a phagocytic receptor for a broad range of pathogens, including bacteria and fungi, facilitating their engulfment and clearance.
• Serves as a host cell receptor for viral entry; it interacts with Dengue virus envelope protein E and Hepatitis B virus envelope protein, contributing to viral internalization.
• Localizes predominantly to the endosome membrane and cell membrane, enabling dynamic cycling between the cell surface and endocytic compartments.
• Contains multiple extracellular C-type lectin domains (CTLDs) and a fibronectin type II domain, characteristic of the mannose receptor family, and requires calcium for ligand binding.
• Undergoes alternative splicing, producing isoforms that may modulate receptor function and ligand specificity.
• Key post-translational modifications include signal peptide cleavage, disulfide bond formation, and N-linked glycosylation, as identified by direct protein sequencing.
• The protein has a calculated molecular weight of approximately 166 kDa and is primarily expressed on tissue macrophages, dendritic cells, and certain endothelial cells.

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular domain (aa 19–1389), which may offer broad epitope coverage for detecting surface CD206.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding, especially in macrophage-rich samples.
• Because the receptor is recycled, avoid prolonged incubation at 37 °C that may lead to internalization; stain at 4 °C or on ice to preserve surface labeling.
• Validate antibody performance in your experimental system using known positive (e.g., M2 macrophages) and negative (e.g., monocytes) controls before quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a curated portfolio of high-quality IVD raw materials, including monoclonal antibodies and conjugated reagents. This Rabbit anti-Human CD206/MMR antibody addresses macrophage biology and infectious disease research needs, and we can supply it alongside validated pairs, bulk ancillary reagents, and customized sourcing for uncommon targets. As your procurement partner, we streamline access to critical reagents, helping you move from discovery to clinical application efficiently.