Rabbit anti-Human CD204 mAb for FC - P21757

Core Product Specifications and Parameters

Biological Background: CD204 Function and Localization

• CD204, also known as macrophage scavenger receptor types I and II (MSR1 or SCARA1), is a transmembrane receptor encoded by the MSR1 gene. It is a member of the scavenger receptor class A family and mediates endocytosis and phagocytosis of a wide range of ligands.
• Recognizes and internalizes modified low-density lipoproteins, including acetylated LDL (AcLDL) and oxidized LDL (OxLDL), thereby promoting cholesterol uptake and contributing to foam cell formation during atherogenesis. Related references: PMID:2251254 PMID:34252459
• Binds a variety of pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs), such as bacterial cell wall components and apoptotic cell debris, supporting macrophage clearance in innate immune defense. Related references: PMID:10722588
• Upon stimulation by CpG DNA, mediates activation of LYN kinase, which phosphorylates SYK, subsequently recruiting it to endosomal TLR9 to enhance TLR9 signaling pathways. Related references: PMID:29057947 PMID:40980882
• Three major isoforms (I, II, III) are generated by alternative splicing. Isoforms I and II are widely expressed in the liver, placenta, brain, and monocyte-derived macrophages, while isoform II lacks the ability to internalize acetylated LDL.
• Located on the cell membrane with a signal-anchor transmembrane domain; structurally features collagen-like, coiled-coil, and cysteine-rich domains, and undergoes post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 77‑451 of human CD204, which includes extracellular domains; the resulting monoclonal antibody likely targets an extracellular epitope, making it suitable for live-cell flow cytometry.
• For flow cytometry (FC) with this conjugate, consider titrating the antibody to determine optimal concentration using relevant human macrophage or monocyte cell lines (e.g., THP‑1, U937) and appropriate isotype controls.
• Validate staining specificity by comparing signal to cells not expressing CD204 or by pre‑blocking with an unconjugated anti‑CD204 antibody; note that CD204 expression can be modulated by inflammatory stimuli.

CamelBio: Your One-Stop Sourcing Bridge

For research on macrophage scavenger receptors and atherosclerosis, CamelBio delivers this Rabbit anti‑Human CD204 mAb as part of a broader suite of IVD raw‑material solutions. Our ready‑to‑use monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents accelerate assay development, while our customized sourcing services address even the most challenging targets.