Rabbit anti-Human CD201/EPCR Monoclonal Antibody for Flow Cytometry - Q9UNN8

Core Product Specifications and Parameters

Biological Background: CD201/EPCR Function and Localization

• The product targets human CD201/EPCR, encoded by the PROCR gene, also known as EPCR, CCCA, and CCD41.
• The protein is officially designated as endothelial protein C receptor and alternatively called activated protein C receptor (APC receptor).
• Core function: binds activated protein C and enhances its activation by the thrombin-thrombomodulin complex, critically regulating the protein C anticoagulant pathway and blood coagulation.
• Subcellular localization: primarily a membrane protein; it is a type I transmembrane receptor.
• Tissue specificity: strongly expressed in endothelial cells of arteries and veins in heart and lung, less intensely in capillaries of lung and skin, and undetectable in small vessels of liver and kidney.
• Structural features: contains a signal peptide, extracellular domain with disulfide bonds, a transmembrane helix, and a cytoplasmic tail; classified as a glycoprotein.
• Keywords from UniProt: Blood coagulation, Hemostasis, Membrane, Receptor, Transmembrane, Transmembrane helix, Signal, Glycoprotein, Disulfide bond.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 18–210), so the antibody likely recognizes a surface‑accessible epitope—suitable for live‑cell flow cytometry without permeabilization.
• Direct conjugation to simplifies staining protocols and reduces background; ensure proper laser and filter configuration for detection (excitation/emission similar to Alexa Fluor 594).
• Confirm CD201/EPCR expression in relevant human endothelial cells (e.g., HUVECs) or cell lines; titrate the antibody to optimize signal‑to‑noise ratio.
• Use appropriate isotype controls and Fc receptor blocking to assess specificity; validate under your experimental conditions and consider that expression may vary between vessel types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers to access critical raw materials like this anti‑CD201/EPCR monoclonal antibody—essential for coagulation and hemostasis assay development. Our comprehensive supply chain offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets, supporting your workflow from early discovery to clinical application.