Rabbit anti-Human CD200R Monoclonal Antibody for Flow Cytometry - Q8TD46

Core Product Specifications and Parameters

Biological Background: CD200R Function and Localization

• CD200R1 (Cell surface glycoprotein CD200 receptor 1) is an inhibitory receptor for the CD200/OX2 cell surface glycoprotein. It limits inflammation by suppressing pro-inflammatory molecules like TNF, interferons, and iNOS upon ligand engagement.
• The receptor also binds the HHV-8 K14 viral CD200 homolog with similar affinity, suggesting a role in viral immune evasion.
• CD200R1 is expressed primarily on hematopoietic cells: granulocytes, monocytes, most T-cells, neutrophils, basophils, and subsets of NK, NKT, and B-cells. It is also found in tissues like bone marrow, lymph nodes, spleen, lung, liver, spinal cord, and kidney.
• At the subcellular level, CD200R1 exists as both a cell membrane protein and a secreted isoform due to alternative splicing.
• The protein is a transmembrane glycoprotein with a signal peptide, disulfide bonds, and a molecular weight around 39 kDa (canonical isoform), though multiple isoforms (19–39 kDa) have been reported.
• Key biological keywords: 3D-structure, Alternative splicing, Disulfide bond, Glycoprotein, Host-virus interaction, Receptor, Secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 29-240) of human CD200R, which includes the ligand-binding region. This design favors recognition of the native cell surface receptor; performing flow cytometry on live, unfixed cells is recommended to preserve epitope conformation.
• Given the existence of multiple isoforms (19–39 kDa), consider including molecular weight markers or isoform-specific controls in any Western blot or SDS-PAGE validation, though this antibody is validated for FC.
• The dye is photostable and bright; use standard red laser (633–647 nm) for detection. Include Fc receptor blocking and live/dead discrimination in flow cytometry panels to minimize nonspecific binding, especially when working with granulocyte or monocyte populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. This anti-CD200R antibody, targeting a key immune checkpoint receptor, supports immuno-oncology and inflammation diagnostic development. From concept to clinic, we streamline procurement of high-quality reagents to accelerate your assay pipeline.