Rabbit anti-Human CD200R Monoclonal Antibody for Flow Cytometry - Q8TD46

Core Product Specifications and Parameters

Biological Background: CD200R Function and Localization

• CD200R1 (OX2R) is a cell surface glycoprotein receptor that binds to CD200 (OX2), functioning as an inhibitory receptor to limit inflammation.
• It inhibits expression of pro-inflammatory molecules such as TNF, interferons, and iNOS upon stimulation; it also interacts with HHV-8 K14 viral homolog with similar affinity.
• Multiple isoforms exist due to alternative splicing, yielding predicted protein weights of approximately 19, 21, 37, and 39 kDa; the mature protein is glycosylated and contains disulfide bonds.
• Subcellular localization includes cell membrane and secreted forms; it is expressed on various immune cells, including granulocytes, monocytes, T-cells, neutrophils, basophils, and subsets of NK, NKT, and B-cells.
• Tissue expression includes bone marrow, lymph nodes, spleen, lung, liver, spinal cord, and kidney, with protein detected in monocyte-derived dendritic and mast cells.
• Keywords highlight its role in host-virus interaction and proteomics identification, and it possesses a transmembrane helix and signal peptide.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 29–240 of human CD200R, representing the extracellular domain; this design favors recognition of native surface receptor for flow cytometry applications.
• For flow cytometry using this conjugate, we recommend titrating the antibody on the target cell type to determine the optimal staining concentration.
• Include appropriate controls such as isotype-matched antibody and fluorescence-minus-one (FMO) controls to ensure specificity.
• Pre-block Fc receptors on human PBMCs or cell lines to reduce nonspecific binding, and confirm cell viability before acquisition.
• Since the recognized epitope lies in the extracellular region, permeabilization is not required for surface staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this CD200R clone, and bulk ancillary reagents, facilitating your assay development from concept to clinic. With expertise in immunology and inflammation targets, CamelBio supports diagnostic manufacturers with rare target sourcing and technical consultation, ensuring reliable supply and performance.