Rabbit anti-Human CD200/OX2 Monoclonal Antibody for Flow Cytometry - P41217

Core Product Specifications and Parameters

Biological Background: CD200 Function and Localization

• CD200, also known as OX-2 membrane glycoprotein, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, implicated in immune regulation.
• It functions as a costimulatory molecule for T-cell proliferation, enhancing T-cell responses.
• CD200 may regulate myeloid cell activity across various tissues, influencing myeloid cell functions.
• The protein is localized on the cell membrane, featuring a single-pass transmembrane helix and an extracellular immunoglobulin-like domain.
• Alternative splicing generates multiple isoforms of CD200, contributing to functional diversity.
• Post-translational modifications include glycosylation and the formation of disulfide bonds, which stabilize its structure.
• The molecular weight of CD200 is approximately 31–32 kDa, with variations due to glycosylation and isoforms.

Experimental Guidance and Technical Tips

• The antibody's immunogen covers the extracellular domain (amino acids 31–232), making it suitable for detecting native surface CD200 by flow cytometry.
• As a directly conjugated antibody, no secondary detection reagent is needed; consider appropriate isotype controls for flow cytometry experiments.
• Validate the antibody's reactivity in human sample systems, as cross-reactivity is limited to human.
• Due to glycosylation and alternative splicing, CD200 may exhibit multiple bands in Western blotting; if adapting for other applications, consider molecular weight variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a complete range of IVD raw materials, including this CD200 monoclonal antibody critical for immune regulation research and diagnostic development. From validated monoclonal antibodies and bulk ancillary reagents to rare target sourcing, we offer a streamlined procurement solution to support your assay development needs. Our one-stop service model helps diagnostic manufacturers and labs efficiently bridge the gap from concept to clinical application.