Rabbit anti-Human CD2 Monoclonal Antibody for Flow Cytometry - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen, also known as LFA-2, SRBC receptor, and T11/Leu-5, belonging to the immunoglobulin superfamily. It mediates cell adhesion through interactions with its ligands.
• It interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types, playing a critical role in T-cell activation.
• The cytoplasmic domain of CD2 is implicated in signal transduction, contributing to T-cell triggering and costimulatory functions.
• Expressed on T lymphocytes and natural killer cells; at the protein level, it is specifically detected in natural killer cells.
• CD2 is a single-pass type I membrane protein with two extracellular immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail. The extracellular domain contains disulfide bonds and N-glycosylation sites.
• The CD2 gene is located on chromosome 1p13.1 and encodes a precursor with a signal peptide. Its molecular weight is approximately 39 kDa, consistent with the mature glycoprotein form.
• Keywords assigned to CD2 include cell adhesion, immunoglobulin domain, transmembrane, glycoprotein, disulfide bond, and signal, reflecting its structural and functional features.

Experimental Guidance and Technical Tips

• This antibody is designed for flow cytometry (FC) with human samples. For surface staining, use live unfixed cells to preserve the native conformation of the CD2 extracellular epitope.
• Consider titrating the antibody to determine the optimal staining concentration for your specific experimental setup, ensuring a high signal-to-noise ratio.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to accurately gate CD2-positive populations.
• If performing intracellular staining, validate the fixation and permeabilization protocol, as it may affect the recognition of the recombinant immunogen-derived epitope.
• For multicolor flow cytometry panels, check for potential spectral overlap with other fluorophores and perform compensation as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined single-window for IVD raw-material procurement. This Rabbit anti-Human CD2 monoclonal antibody supports immunophenotyping and T-cell research, and we can supply additional validated antibody pairs, optimized clones, and bulk ancillary reagents. Our vertical integration ensures traceable, scalable sourcing from concept to clinic, covering rare-target antibody development and custom conjugation services.