Rabbit anti-Human CD2 mAb - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen also known as LFA-2, the erythrocyte receptor, and the rosette receptor. It is a member of the immunoglobulin superfamily and functions as a cell adhesion molecule.
• The extracellular region of CD2 binds to CD58 (LFA-3) on antigen-presenting cells and to CD48/BCM1, facilitating intercellular adhesion between T-cells and other cell types, which is critical for immune synapse formation.
• Beyond adhesion, CD2 acts as a co-stimulatory molecule; its cytoplasmic domain transmits signals that synergize with T-cell receptor (TCR) activation, contributing to T-cell triggering and proliferation.
• CD2 is predominantly localized to the cell membrane of T-cells and natural killer (NK) cells, with high expression in circulating lymphocytes and thymocytes.
• The protein contains a single transmembrane helix, two extracellular immunoglobulin-like domains, and a cytoplasmic tail involved in signal transduction.
• Post-translational modifications include N-linked glycosylation and the presence of disulfide bonds that stabilize the immunoglobulin folds, which are typical of this protein family.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular segment (amino acids 25–209) of human CD2, suggesting that the antibody recognizes an epitope accessible on live cells; consider it for surface staining in flow cytometry assays.
• Since this antibody is fluorophore-conjugated (), direct immunofluorescence staining protocols can be applied without a secondary detection step; titrate the antibody to determine the optimal concentration for your cell type.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls when setting up multi-color panels to ensure accurate gating of CD2-positive populations.
• For intracellular detection, verify that the fixation and permeabilization method does not alter the epitope; the immunogen design supports surface staining, so intracellular validation may require additional optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic and research laboratories with reliable access to high-quality IVD raw materials, including validated antibodies for immune cell profiling. This rabbit anti-human CD2 monoclonal antibody exemplifies our commitment to offering ready-to-use reagents for flow cytometry and T-cell/NK cell studies. Beyond single products, CamelBio provides a comprehensive portfolio—from antibody pairs and bulk ancillary reagents to custom monoclonal/polyclonal development—all supported by end-to-end technical and regulatory guidance to accelerate your projects from concept to clinic.