Rabbit anti-Human CD1d Monoclonal Antibody for Flow Cytometry - P15813

Core Product Specifications and Parameters

Biological Background: CD1d Function and Localization

• CD1d is an antigen-presenting glycoprotein that binds self and non-self glycolipids.
• It presents glycolipids to T-cell receptors on natural killer T-cells, linking innate and adaptive immunity.
• The protein traffics through multiple membrane compartments: cell membrane (basolateral), endosomes, lysosomes, and endoplasmic reticulum.
• Tissue expression includes cortical thymocytes, certain T-cell leukemias, and various other tissues.
• Structurally, CD1d contains an immunoglobulin domain and conserved disulfide bonds typical of MHC class I-like molecules.

Experimental Guidance and Technical Tips

• For flow cytometry, validate the antibody using a known CD1d-expressing cell line as a positive control and an isotype control to assess non-specific binding.
• Consider using freshly isolated peripheral blood mononuclear cells or thymocytes to preserve cell surface CD1d expression.
• Bright signal enables strong separation, but avoid fluorophore-conjugated antibody cocktails that may cause spectral overlap.
• Fixation and permeabilization steps may alter CD1d staining; test both surface and intracellular protocols if needed.

CamelBio: Your One-Stop Sourcing Bridge

This CD1d monoclonal antibody supports NKT cell and glycolipid presentation research. CamelBio delivers reliable IVD raw materials with flexible supply solutions, including validated antibody pairs, optimized bulk monoclonals/polyclonals, and ancillary reagents. Our one-stop sourcing model streamlines procurement for diagnostic developers, from concept to clinic, complemented by technical services for customized rare-target reagent development.