Rabbit anti-Human CD1d Monoclonal Antibody for Flow Cytometry - P15813

Core Product Specifications and Parameters

Biological Background: CD1d Function and Localization

• CD1d (also known as R3G1) is an antigen-presenting glycoprotein that belongs to the CD1 family of non-classical MHC class I molecules.
• It binds self and non-self glycolipids, presenting them to T-cell receptors on natural killer T (NKT) cells, playing a critical role in innate immunity.
• The protein contains an immunoglobulin-like domain and a transmembrane region, and is structurally characterized by disulfide bonds and glycosylation.
• CD1d localizes to various cellular membranes, including the cell membrane (specifically basolateral in polarized cells), endosome membrane, lysosome membrane, and endoplasmic reticulum membrane, reflecting its trafficking and antigen-loading compartments.
• Expressed on cortical thymocytes, certain T-cell leukemias, and in various other tissues, indicating a role in thymic selection and peripheral immune surveillance.
• As a member of the CD1 family, CD1d is specifically recognized by invariant NKT cells, bridging innate and adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 20–301 (most of the extracellular domain), likely targeting surface-exposed epitopes suitable for flow cytometry—the validated application.
• Due to its cell surface and intracellular localization, consider using appropriate fixation and permeabilization protocols if performing intracellular flow cytometry staining.
• CD1d is expressed on multiple immune cell types; optimize antibody concentration and incubation conditions for your specific sample to minimize background.
• While validated for FC, other applications (e.g., immunofluorescence) may be explored but require thorough validation in the relevant system.
• Shipment on ice bag ensures cold-chain maintenance; store antibody as recommended, typically at -20°C or 4°C with preservatives.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw materials, including validated antibodies like this anti-CD1d clone for immunology research and assay development. In addition to individual reagents, we offer optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and sourcing assistance for rare-target raw materials—supporting your entire workflow from concept to clinical application.