Rabbit anti-Human CD1c Monoclonal Antibody for FC - P29017

Core Product Specifications and Parameters

Biological Background: CD1c Function and Localization

• CD1c is a member of the CD1 family of glycoproteins, characterized by an immunoglobulin-like domain and involved in lipid antigen presentation to T cells.
• It functions as an antigen-presenting molecule that binds and presents self and non-self lipid and glycolipid antigens to T-cell receptors on natural killer T-cells, playing a role in adaptive immunity.
• CD1c localizes to the cell membrane, as well as endosome and lysosome membranes, where it samples lipid antigens from intracellular compartments.
• The protein is expressed on cortical thymocytes, certain T-cell leukemias, and in various other tissues, making it a marker for dendritic cell subsets (e.g., BDCA1+ myeloid dendritic cells).
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for its structure and function.
• The predicted molecular weight of the mature protein is approximately 38 kDa (37654 Da), with observed bands around 38 kDa depending on glycosylation.

Experimental Guidance and Technical Tips

• This antibody is designed for flow cytometry (FC) and the immunogen corresponds to the extracellular domain, so it is expected to recognize native cell surface CD1c.
• For optimal staining, titrate the antibody to determine the best concentration for your specific cell type and experimental conditions, and consider using appropriate isotype controls and Fc receptor blocking.
• Validate the antibody in your relevant sample system, such as PBMCs or leukemia cell lines, to confirm specificity and sensitivity for your application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this CD1c clone for immune cell analysis. We offer bulk ancillary reagents and rare target sourcing to support your immunology and oncology assay development from concept to clinic.