Rabbit anti-Human CD1B Monoclonal Antibody for FC - P29016

Core Product Specifications and Parameters

Biological Background: CD1b Function and Localization

• CD1b is a type I transmembrane glycoprotein belonging to the CD1 family, which are structurally related to MHC class I molecules and contain an immunoglobulin-like domain.
• It functions as an antigen-presenting molecule that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
• CD1b localizes to the cell membrane, as well as to endosome and lysosome membranes, facilitating lipid antigen processing and presentation in endocytic compartments.
• Expression is reported on cortical thymocytes, certain T-cell leukemias, and other tissues, consistent with a role in immune surveillance.
• The protein undergoes alternative splicing, yielding isoforms with molecular weights of approximately 37 kDa and 30 kDa.
• Key structural features include disulfide bonds, glycosylation sites, a signal peptide, and a single transmembrane helix, as well as a resolved 3D structure.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular region (amino acids 18-303), which should target the native conformation of CD1b on the cell surface.
• This antibody is directly conjugated to , making it ready-to-use for flow cytometry analysis without the need for secondary reagents.
• Titrate the antibody in your specific cell system to determine the optimal staining concentration, and include appropriate positive and negative controls (e.g., CD1b-expressing vs. non-expressing cells).
• Note that alternative isoforms may exist; confirm isoform specificity if required for your experimental design.
• Maintain cell surface integrity during sample preparation to preserve membrane-bound CD1b detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material sourcing, providing validated antibody pairs, optimally conjugated monoclonal antibodies like this anti-CD1b clone, and bulk ancillary reagents. We support diagnostic developers and researchers studying lipid antigen presentation and T-cell biology, offering tailored procurement solutions from early concept through clinical application.