Rabbit anti-Human CD185/CXCR5 Monoclonal Antibody for Flow Cytometry - P32302

Core Product Specifications and Parameters

Biological Background: CXCR5 Function and Localization

• CD185/CXCR5 is a C-X-C chemokine receptor that specifically binds the chemokine BLC (B-lymphocyte chemoattractant), also known as CXCL13.
• It plays a critical role in directing B-cell migration into B-cell follicles of spleen and Peyer patches, but not into those of mesenteric or peripheral lymph nodes.
• The receptor is a member of the G protein-coupled receptor family and features seven transmembrane domains, a hallmark of chemokine receptors.
• CXCR5 is predominantly expressed on mature B-cells and Burkitt lymphoma cells, highlighting its involvement in B-cell biology and lymphomagenesis.
• It may have a regulatory function in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation.
• The protein is located on the cell membrane, consistent with its role as a transmembrane signaling receptor.
• Post-translational modifications include glycosylation and disulfide bonds, which are typical for GPCRs and essential for proper folding and function.
• Alternative splicing of CXCR5 yields multiple isoforms, potentially modulating receptor activity in different contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-47), making it suitable for generating antibodies that recognize the receptor on live cells in flow cytometry.
• Since the antibody is validated for FC, it likely targets a conformational epitope on the cell surface; consider optimizing staining protocols with appropriate Fc receptor blocking and titration.
• Confirm specificity using positive control cells such as Burkitt lymphoma cell lines and negative controls lacking CXCR5 expression.
• For intracellular staining, permeabilization may affect epitope recognition; validate in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a one-stop sourcing solution for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. In the field of CD185/CXCR5 research, we supply validated antibody pairs, optimized monoclonal antibodies like this anti-CXCR5 clone, bulk ancillary reagents, and rare target raw-material sourcing to support B-cell lymphoma studies and immunological assay development. Our expertise ensures seamless procurement and dedicated support for your diagnostic projects.