Rabbit anti-Human CD185/CXCR5 Monoclonal Antibody for FC - P32302

Core Product Specifications and Parameters

Biological Background: CD185/CXCR5 Function and Localization

• CD185/CXCR5, also known as Burkitt lymphoma receptor 1 (BLR1) and monocyte-derived receptor 15 (MDR-15), is a cytokine receptor belonging to the G protein-coupled receptor family.
• It functions as a receptor for B-lymphocyte chemoattractant (BLC), playing a critical role in directing B-cell migration into B-cell follicles of the spleen and Peyer patches.
• It is implicated in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation, suggesting a regulatory role in B-cell malignancies.
• The protein is localized on the cell membrane and is predicted to contain seven transmembrane domains, characteristic of GPCRs.
• Post-translational modifications include disulfide bond formation and glycosylation, which are common among chemokine receptors.
• Expression is primarily observed in mature B-cells and Burkitt lymphoma cells.
• Alternative splicing has been reported, yielding different isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-47) of human CD185/CXCR5, suggesting the antibody may recognize an extracellular epitope suitable for flow cytometry.
• For flow cytometry, consider using human B-cell lines or Burkitt lymphoma cell lines (e.g., Raji, Daudi) as positive controls, based on tissue specificity data.
• Since the antibody is directly conjugated to , it can be used for direct immunofluorescence staining without a secondary antibody; titrate to determine optimal staining concentration.
• Cross-reactivity is reported for human; appropriate controls should be included when testing other species.
• For blocking or neutralization studies, note that the immunogen is limited to the first 47 amino acids, and biological effects may require receptor engagement validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive range of IVD raw materials, including this -conjugated anti-CD185/CXCR5 monoclonal antibody. Our portfolio covers bulk monoclonals, validated antibody pairs, and ancillary reagents for flow cytometry and immunoassay development, streamlining your path from concept to clinic. Reach out to explore sourcing options for rare targets or custom antibody production.