Rabbit Anti-Human CD177 Monoclonal Antibody () for Flow Cytometry - Q8N6Q3

Core Product Specifications and Parameters

Biological Background: CD177 Function and Localization

• CD177 (also designated HNA-2a, NB1 GP, PRV-1) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on a subset of mature human neutrophils.
• It associates with the beta-2 integrin heterodimer ITGAM/CD11b–ITGB2/CD18 to mediate activation of TNF-primed neutrophils, including degranulation and superoxide production (PubMed:21193407).
  Related references: PMID:21193407
• CD177 acts as a cell surface receptor for mature proteinase 3 (PRTN3), enabling PRTN3 display on neutrophils. This interaction may enhance endothelial junctional integrity during neutrophil diapedesis (PubMed:17244676, PubMed:18462208, PubMed:23202369).
  Related references: PMID:17244676 PMID:18462208 PMID:23202369
• The protein promotes neutrophil adhesion over migration by attenuating chemokine signaling and preventing beta-2 integrin internalization (PubMed:28807980).
  Related references: PMID:28807980
• Heterophilic binding to PECAM1 on endothelial cells facilitates neutrophil transendothelial migration in vitro, although CD177 appears dispensable for neutrophil recruitment during bacterial infection in vivo (PubMed:17580308, PubMed:23461681).
  Related references: PMID:17580308 PMID:23461681
• CD177 is highly expressed in normal bone marrow and is upregulated on granulocytes from patients with polycythemia vera (PV) and essential thrombocythemia (ET), serving as a disease marker (PubMed:10753836, PubMed:12377969).
  Related references: PMID:10753836 PMID:12377969
• Expression begins at the metamyelocyte stage during neutrophil differentiation and the percentage of CD177-positive neutrophils varies widely among healthy individuals (PubMed:17244676, PubMed:18462208, PubMed:24926686, PubMed:27227454).
  Related references: PMID:17244676 PMID:18462208 PMID:24926686 PMID:27227454
• Subcellularly, CD177 localizes to the plasma membrane, membrane rafts, secretory vesicles, cytoplasmic granule membranes, and lamellipodia, consistent with roles in trafficking and secretion.

Experimental Guidance and Technical Tips

• The conjugate is suitable for direct flow cytometric detection of CD177 on human neutrophil populations. Consider using appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• The immunogen comprises the extracellular domain (amino acids 22–407), suggesting the antibody recognizes native CD177 on live cells without permeabilization.
• Because CD177 is expressed only by a neutrophil subset, include surface markers (e.g., CD16, CD66b) to properly gate neutrophils and assess the CD177-positive fraction.
• Titrate the antibody to determine the optimal concentration for your specific flow cytometry assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and sourcing services for the global diagnostics community. This anti‑CD177 monoclonal antibody exemplifies our commitment to providing validated reagents for neutrophil research and inflammation assays. Beyond individual antibodies, we supply antibody pairs, bulk ancillary reagents, and custom procurement for rare targets—all supported by technical consulting to accelerate your development from concept to clinic.