Rabbit anti-Human CD177 Monoclonal Antibody for FC - Q8N6Q3

Core Product Specifications and Parameters

Biological Background: CD177 Function and Localization

• CD177 (also known as HNA-2a, NB1 GP, PRV-1) is a GPI-anchored glycoprotein and a member of the immunoglobulin superfamily, expressed on the surface of neutrophils.
• In association with the beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, CD177 mediates TNF-primed neutrophil degranulation and superoxide production. Related references: PMID:21193407
• By preventing beta-2 integrin internalization and attenuating chemokine signaling, CD177 favors neutrophil adhesion over migration. Related references: PMID:28807980
• Heterophilic interaction with PECAM1 on endothelial cells supports neutrophil transendothelial migration in vitro, though CD177 appears dispensable for neutrophil recruitment during bacterial infection in vivo. Related references: PMID:17580308 PMID:23461681
• CD177 acts as a receptor for the mature form of protease PRTN3, enabling its display on the neutrophil surface and potentially enhancing endothelial junctional integrity during diapedesis. Related references: PMID:17244676 PMID:18462208 PMID:23202369
• The protein localizes to the cell membrane, membrane rafts, cytoplasmic granule membrane, and lamellipodia; a secreted form also exists.
• Highly expressed in normal bone marrow and during neutrophil differentiation from the metamyelocyte stage onward, with variable expression in mature neutrophils across individuals. Upregulated in granulocytes from patients with polycythemia vera and essential thrombocythemia.
• Comprises alternative splicing, cell adhesion, GPI-anchor, glycoprotein, and innate immunity keywords in UniProtKB.

Experimental Guidance and Technical Tips

• For direct flow cytometry, the pre-conjugated label eliminates the need for secondary antibody steps; titrate the antibody to determine the optimal staining index for your specific cell population.
• Since the immunogen covers the extracellular domain (aa 22-407), the antibody likely recognizes an epitope in the native protein; consider using knockout cell lines or competitive blocking with recombinant CD177 to confirm specificity.
• Validate the antibody in your experimental system, as neutrophil CD177 expression varies among donors and may be influenced by activation state or disease conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement with a comprehensive portfolio, including this conjugated CD177 antibody for neutrophil and inflammation research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development—from concept to clinic. Partner with CamelBio for efficient access to the raw materials that power your diagnostic innovations.